【演者】竹内 力先生（Postdoctoral Fellow, Green Center for Reproductive Biology Sciences, University of Texas Southwestern ）【演題】Transcription factor regulatory networks during human cardiac differentiation

大学院特別講義・第57回システム発生・再生医学分野セミナー 本学ハイブリッド形式にて開催します

日時：令和8年7月28日（火）17:00-19:00 要旨：Transcription factors (TFs) control the timing of gene programs during lineage commitment, and dysfunction of this regulatory network leads to developmental diseases such as congenital heart diseases. However, systematically defining the dynamic activities of the human genome's nearly 2000 TFs during development remains challenging. In this talk, I will discuss our recent findings on a systematic dissection of the gene regulatory network during cardiac differentiation. I used Perturb-Seq to map the transcriptional impact of nearly all human TFs during cardiomyocyte differentiation. Our results suggest that developmental regulatory networks are distributed across highly connected hub TFs that are dynamic across lineage and time, rather than organizing into top-down hierarchies directed by "master regulators". Building on these regulatory interactions, we construct a deep learning transformer model to accurately predict perturbed TFs driving altered regulatory networks in patient-derived transcriptomes. Together, our results define the regulatory network architecture of human lineage specification and provide a platform for predicting TF function and interpreting disease mechanisms.